用于临床相关生物测定的甲状腺培养细胞系统的优化。

Refinement of a thyroid cultured cell system used in clinically relevant bioassays.

作者信息

Ambesi-Impiombato F S, Miani P, Piemonte M, Perrella G, Curcio F

机构信息

Istituto di Patologia Clinica e Sperimentale, University of Udine Medical School, Italy.

出版信息

Cytotechnology. 1993;11 Suppl 1:S120-2.

PMID:7763738

Abstract

Thyroid cultured cells are now used worldwide in clinical bioassays of TSH and of thyroid autoantibodies. Having originally developed the thyroid cell cultures (Ambesi-Impiombato et al. 1980) from rat glands in our laboratory, we now aim to improve the system, moving in two directions: a) TSH-independent mutants have been produced and characterized, which can be used in clinical bioassays without "starvation" from the hormone. b) Human cultures have been attempted using our experience with rat cells, as well as innovative strategies. Preliminary results now indicate that human normal differentiated cells may be available for clinical studies in vitro, when species-specific differences may be critical.

摘要

甲状腺培养细胞目前在全球范围内用于促甲状腺激素（TSH）和甲状腺自身抗体的临床生物测定。我们实验室最初从大鼠腺体中培养出甲状腺细胞（安贝西 - 因皮奥姆巴托等人，1980年），现在我们旨在从两个方向改进该系统：a）已产生并鉴定了不依赖TSH的突变体，可用于临床生物测定，而无需因缺乏该激素而进行“饥饿处理”。b）利用我们在大鼠细胞方面的经验以及创新策略尝试进行人类细胞培养。初步结果表明，当物种特异性差异可能至关重要时，人类正常分化细胞可能可用于体外临床研究。