使用超临界流体萃取法分离生物分子。
Separation of biomolecules using supercritical fluid extraction.
作者信息
Lucien F P, Liong K K, Cotton N J, Macnaughton S J, Foster N R
机构信息
School of Chemical Engineering and Industrial Chemistry, University of New South Wales, Kensington, Australia.
出版信息
Australas Biotechnol. 1993 May-Jun;3(3):143-7.
Supercritical fluids are increasingly being used as a replacement for more conventional organic solvents in the extraction of biomolecules from a range of matrices. Supercritical fluid extraction of essential fatty acids from fish oils is discussed. Supercritical CO2 was used to fractionate two fatty acids, EPA and DHA from fish oil ethyl esters. EPA and DHA were obtained with a purity of 58% and 67% respectively from Sardine oil with an original composition of 17% and 12%.
在从一系列基质中提取生物分子时，超临界流体正越来越多地被用作更传统有机溶剂的替代品。本文讨论了从鱼油中提取必需脂肪酸的超临界流体萃取方法。使用超临界二氧化碳从鱼油乙酯中分离出两种脂肪酸，即二十碳五烯酸（EPA）和二十二碳六烯酸（DHA）。从原始组成为17% EPA和12% DHA的沙丁鱼油中分别获得了纯度为58%的EPA和67%的DHA。
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