Chen D C, Yang B C, Chow C W, Kuo T T
Institute of Botany, Academic Sinica, Taipei, Taiwan.
J Biotechnol. 1993 Jun;29(3):329-34. doi: 10.1016/0168-1656(93)90064-t.
We describe here a simple method for analyzing the stability of recombinant yeast. The mouse salivary alpha-amylase gene was used in this system as a marker since the stability of recombinant yeast can be detected easily by a halo zone-for-ming assay on starch-supplemented plates. We used this method to evaluate the stability of recombinant yeast harbouring a 2 mu directed episomal plasmid and of yeast harbouring the r-DNA directed integrative vector. Our results demonstrated clearly that the alpha-amylase gene was a convenient and reliable marker for evaluating the stability of recombinant yeasts in long-term cultivation.
我们在此描述一种分析重组酵母稳定性的简单方法。在该系统中使用小鼠唾液α-淀粉酶基因作为标记，因为通过在补充淀粉的平板上进行形成晕圈区域的检测，可以轻松检测重组酵母的稳定性。我们使用该方法评估携带2μm定向附加体质粒的重组酵母和携带r-DNA定向整合载体的酵母的稳定性。我们的结果清楚地表明，α-淀粉酶基因是评估重组酵母在长期培养中稳定性的方便且可靠的标记。
