Benkerroum N, Ghouati Y, Sandine W E, Tantaoui-Elaraki A
Départment de Microbiologie, Alimentaire et de Biotechnologie, Rabat Instituts, Morocco.
Lett Appl Microbiol. 1993 Aug;17(2):78-81. doi: 10.1111/j.1472-765x.1993.tb00376.x.
Two simple techniques were developed to demonstrate bactericidal activity of bacteriocins. Both were based on allowing a lawn of indicator strain to grow first, then exposing the lawn to bacteriocin-containing cell-free supernatants in a well cut in the seeded agar lawn or by inoculating the bacteriocin-producing strain onto the indicator lawn. Lysis of cells of the indicator strain resulted in a clear zone. These techniques may be adapted to test antimicrobial substances other than bacteriocins and to help to determine their modes of action.
开发了两种简单技术来证明细菌素的杀菌活性。这两种技术均基于先让指示菌株的菌苔生长，然后将该菌苔暴露于接种琼脂平板上切出的孔中含细菌素的无细胞上清液，或通过将产生细菌素的菌株接种到指示菌苔上。指示菌株细胞的裂解会导致形成清晰的抑菌圈。这些技术可用于测试除细菌素以外的抗菌物质，并有助于确定其作用方式。
