Yamaguchi K, Ueda M, Udaka S
Life Science Research Institute, Snow Brand Milk Products Co., Ltd., Tochigi, Japan.
Biosci Biotechnol Biochem. 1993 Aug;57(8):1384-6. doi: 10.1271/bbb.57.1384.
To investigate the role of signal peptides in extracellular production in Bacillus brevis, the Bacillus stearothermophilus alpha-amylase signal peptide was systematically altered and the effects were analyzed in B. brevis. Efficient extracellular production of the amylase in B. brevis was accomplished either by introducing point mutations at positions between -6 and -4 or by replacing the whole signal peptide with that of B. brevis middle wall protein.
为了研究信号肽在短短芽孢杆菌胞外产物合成中的作用，对嗜热脂肪芽孢杆菌α淀粉酶信号肽进行了系统改造，并在短短芽孢杆菌中分析其效果。在短短芽孢杆菌中高效胞外合成淀粉酶，可通过在-6至-4位之间引入点突变，或用短短芽孢杆菌中层壁蛋白的信号肽替换整个信号肽来实现。
