Reff M E
IDEC Pharmaceuticals Corporation, San Diego.
Curr Opin Biotechnol. 1993 Oct;4(5):573-6. doi: 10.1016/0958-1669(93)90079-c.
Successful high-level expression of recombinant immunoglobulins has now been reported in several different types of mammalian cells. This has been achieved using random integration of immunoglobulin genes co-linked with a drug-resistance gene into cellular DNA. After gene integration, increases in immunoglobulin-gene expression are obtained by selection for increases in expression of the drug-resistance marker. Expression levels attained by this method are several fold higher than those produced by a good hybridoma. Soon, site-specific homologous recombination may replace random integration as the favored methodology for initial expression of all proteins in mammalian cells, including immunoglobulins.
目前已有报道称，在几种不同类型的哺乳动物细胞中成功实现了重组免疫球蛋白的高水平表达。这是通过将与耐药基因共连接的免疫球蛋白基因随机整合到细胞DNA中来实现的。基因整合后，通过选择耐药标记物表达的增加来提高免疫球蛋白基因的表达。通过这种方法获得的表达水平比优质杂交瘤产生的表达水平高几倍。很快，位点特异性同源重组可能会取代随机整合，成为在哺乳动物细胞中包括免疫球蛋白在内的所有蛋白质初始表达的首选方法。
