Fukuda H, Fujii T, Daimon H, Iwata M, Ogawa T, Tanase S, Morino Y
Department of Applied Microbial Technology, Kumamoto Institute of Technology, Japan.
Biosci Biotechnol Biochem. 1993 Sep;57(9):1599-601. doi: 10.1271/bbb.57.1599.
A cytochrome P450 was purified from microsomes of Rhodotorula minuta. The optical spectrum of the purified cytochrome was characteristic of a low-spin ferric heme protein. Isovalerate caused a type I spectral change in it. The amino-terminal sequence of the cytochrome was different from those of other known microsomal cytochrome P450s. These results indicate that the cytochrome, which is tentatively named P450rm, is a novel species of cytochrome P450.
从微小红酵母微粒体中纯化出一种细胞色素P450。纯化后的细胞色素的光谱是低自旋铁血红素蛋白的特征光谱。异戊酸使其产生I型光谱变化。该细胞色素的氨基末端序列与其他已知的微粒体细胞色素P450不同。这些结果表明，这种暂时命名为P450rm的细胞色素是一种新型细胞色素P450。
