Capiaumont J, Legrand C, Gelot M A, Straczek J, Belleville F, Nabet P
Laboratoire de Biochimie Médicale du Professeur P. Nabet, Faculté de Médecine, Université de Nancy I, France.
J Biotechnol. 1993 Nov;31(2):147-60. doi: 10.1016/0168-1656(93)90157-i.
The control of hybridoma cell cultures in bioreactors requires the use of convenient indicators to monitor the proliferation of the biomass. In order to select appropriate indications, we followed the variations of several compounds including tumoral markers, polyamines, sialic acids, purine and pyrimidine bases, enzymes and metabolites such as glucose, lactate and amino acids, and the variations of cell density during batch culture. Significant correlations were found between the number of viable cells and alkaline phosphatase, beta-glucuronidase, glucose and lactate measured in the culture medium of hybridoma strains. The correlation calculated from alkaline phosphatase and beta-glucuronidase concentrations in culture medium underestimated cell number. The correlation established with glucose and lactate gave the best indication of cell proliferation in continuous culture with an immobilized cell bioreactor. Finally, the exact quantification of the biomass in these culture conditions can be obtained using the mean of glucose and lactate correlations.
在生物反应器中控制杂交瘤细胞培养需要使用便捷的指标来监测生物量的增殖。为了选择合适的指标，我们跟踪了几种化合物的变化情况，包括肿瘤标志物、多胺、唾液酸、嘌呤和嘧啶碱基、酶以及葡萄糖、乳酸和氨基酸等代谢物，还跟踪了分批培养过程中细胞密度的变化。在杂交瘤菌株的培养基中测得的活细胞数量与碱性磷酸酶、β-葡萄糖醛酸酶、葡萄糖和乳酸之间存在显著相关性。根据培养基中碱性磷酸酶和β-葡萄糖醛酸酶浓度计算出的相关性低估了细胞数量。在固定化细胞生物反应器的连续培养中，与葡萄糖和乳酸建立的相关性能最好地指示细胞增殖情况。最后，利用葡萄糖和乳酸相关性的平均值可在这些培养条件下实现生物量的精确量化。
