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嗜热脂肪芽孢杆菌NS1122A菌株中一种耐热N-氨甲酰-L-氨基酸酰胺水解酶基因的分子克隆与测序

Molecular cloning and sequencing of the gene for a thermostable N-carbamyl-L-amino acid amidohydrolase from Bacillus stearothermophilus strain NS1122A.

作者信息

Mukohara Y, Ishikawa T, Watabe K, Nakamura H

机构信息

Okawara Research Center, Nippon Soda Co., Ltd., Kanagawa, Japan.

出版信息

Biosci Biotechnol Biochem. 1993 Nov;57(11):1935-7. doi: 10.1271/bbb.57.1935.

DOI:10.1271/bbb.57.1935
PMID:7764340
Abstract

The gene for N-carbamyl-L-amino acid amidohydrolase was cloned from Bacillus stearothermophilus strain NS1122A into E. coli. This gene started with a TTG triplet and was predicted to encode a peptide of 409 amino acids, with a calculated molecular weight of 44,248. The deduced amino acid sequence shared moderate homology with that of the corresponding enzyme of Pseudomonas sp. strain NS671.

摘要

N-氨甲酰-L-氨基酸酰胺水解酶基因从嗜热脂肪芽孢杆菌NS1122A菌株克隆到大肠杆菌中。该基因起始于TTG三联体，预计编码一个由409个氨基酸组成的肽段，计算分子量为44,248。推导的氨基酸序列与假单胞菌属NS671菌株相应酶的氨基酸序列具有中等同源性。

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