编码L-立体特异性氨甲酰酶和氨基酰化酶的两个氨基酸酰胺水解酶基因在嗜热脂肪芽孢杆菌的一个共同操纵子中排列。
Two amino acid amidohydrolase genes encoding L-stereospecific carbamoylase and aminoacylase are organized in a common operon in Bacillus stearothermophilus.
作者信息
Batisse N, Weigel P, Lecocq M, Sakanyan V
机构信息
Laboratoire de Biotechnologie, Faculté des Sciences et des Techniques, Université de Nantes, France.
出版信息
Appl Environ Microbiol. 1997 Feb;63(2):763-6. doi: 10.1128/aem.63.2.763-766.1997.
Abstract
The L-carbamoylase gene (amaB) upstream of the previously detected L-aminoacylase gene (amaA) in the Bacillus stearothermophilus NCIB8224 strain was identified in this study. The amaB and amaA genes are cotranscribed as a single mRNA from the same transcriptional start. The two-ama-gene operon is conserved in B. stearothermophilus strains. A cross-activity of L-carbamoylase towards respective substrates for L-aminoacylase supports the hypothesis of a common ancestor for both amino acid amidohydrolase genes.
摘要
在本研究中，我们鉴定了嗜热脂肪芽孢杆菌NCIB8224菌株中先前检测到的L-氨基酰化酶基因（amaA）上游的L-氨基甲酰酶基因（amaB）。amaB和amaA基因从相同的转录起始位点共转录为单一的mRNA。双ama基因操纵子在嗜热脂肪芽孢杆菌菌株中是保守的。L-氨基甲酰酶对L-氨基酰化酶各自底物的交叉活性支持了这两个氨基酸酰胺水解酶基因有共同祖先的假说。
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