棘孢曲霉纤维素酶（FI - CMCase）基因在大肠杆菌中的表达。

Expression of the cellulase (FI-CMCase) gene of Aspergillus aculeatus in Escherichia coli.

作者信息

Ooi T, Minamiguchi K, Kawaguchi T, Okada H, Murao S, Arai M

机构信息

Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture, Japan.

出版信息

Biosci Biotechnol Biochem. 1993 Nov;57(11):1960-1. doi: 10.1271/bbb.57.1960.

DOI:10.1271/bbb.57.1960

PMID:7764343

Abstract

FI-CMCase cDNA of Aspergillus aculeatus was expressed in Escherichia coli by using the tac promoter of E. coli. Transformants of E. coli harboring a plasmid pHEM06 containing mature form FI-CMCase cDNA produced FI-CMCase in the cytoplasm of the cells. The enzyme from E. coli cells was purified to yield 56% and it was immunological identical to that of FI-CMCase purified from A. aculeatus.

摘要

通过使用大肠杆菌的tac启动子，将棘孢曲霉的FI - CMCase cDNA在大肠杆菌中进行表达。携带含有成熟形式FI - CMCase cDNA的质粒pHEM06的大肠杆菌转化子在细胞胞质中产生FI - CMCase。从大肠杆菌细胞中纯化该酶，产率为56%，并且其与从棘孢曲霉中纯化的FI - CMCase具有免疫同一性。