棘孢曲霉纤维素酶（FI-羧甲基纤维素酶）基因在酿酒酵母中的表达。

Expression of the cellulase (FI-CMCase) gene of Aspergillus aculeatus in Saccharomyces cerevisiae.

作者信息

Ooi T, Minamiguchi K, Kawaguchi T, Okada H, Murao S, Arai M

机构信息

Department of Agricultural Chemistry, College of Agriculture, University of Osaka Prefecture, Japan.

出版信息

Biosci Biotechnol Biochem. 1994 May;58(5):954-6. doi: 10.1271/bbb.58.954.

DOI:10.1271/bbb.58.954

PMID:7764982

Abstract

As a step to breed a Saccharomyces cerevisiae strain able to produce ethanol directly from cellulose, we combined cDNA for Aspergillus aculeatus FI-CMCase (FI-carboxymethyl cellulase) with the GAP (glyceraldehyde-3-phosphate dehydrogenase) promoter of S. cerevisiae and used the resultant plasmid, pYEC91, to transform S. cerevisiae. The transformed cells produced active FI-CMCase within the cytoplasm. Western-blot analysis following SDS-polyacrylamide gel electrophoresis demonstrated that the cells contained a peptide having the same molecular mass and immunological identity as A. aculeatus FI-CMCase.

摘要

作为培育一种能够直接从纤维素生产乙醇的酿酒酵母菌株的一个步骤，我们将棘孢曲霉FI - 羧甲基纤维素酶（FI - carboxymethyl cellulase）的cDNA与酿酒酵母的甘油醛 - 3 - 磷酸脱氢酶（GAP，glyceraldehyde-3-phosphate dehydrogenase）启动子相结合，并使用所得质粒pYEC91转化酿酒酵母。转化后的细胞在细胞质内产生了活性FI - 羧甲基纤维素酶。SDS - 聚丙烯酰胺凝胶电泳后的蛋白质免疫印迹分析表明，这些细胞含有一种与棘孢曲霉FI - 羧甲基纤维素酶具有相同分子量和免疫特性的肽。