Jacobsen S E, Shi L, Xin Z, Olszewski N E
Department of Plant Biology, University of Minnesota, St. Paul 55108.
Planta. 1994;192(3):372-8. doi: 10.1007/BF00198573.
The gib1 mutant of tomato (Lycopersicon esculentum Mill.) is deficient in endogenous gibberellins and exhibits phenotypes including extreme dwarfism, reduced germination, and abnormal flower development, which are reversed by the application of gibberellic acid (GA3). Previous work has demonstrated that, in stamens of the gib1 mutant, pollen mother-cell development arrests at the premeiotic G1 stage (Jacobsen and Olszewski 1991, Plant Physiol. 97, 409-414). Following GA3 treatment of developmentally arrested flowers, pollen mother-cell development resumes and is synchronous. The present study examines gibberellin-induced changes in the translatable mRNA populations of developmentally arrested stamens and of vegetative shoots of the gib1 mutant. Following rescue of developmentally arrested stamens by treatment with GA3, we consistently detected increases and decreases in the abundance of 14 and 20 in-vitro translation products, respectively. Some of these changes were first detected 8 h post treatment and therefore represent the first changes observed in stamens whose development has been rescued by GA3 treatment. In vegetative gib1 shoots, the abundance of 13 in-vitro translation products decreased within 6-24 h after GA3 treatment. However, no in-vitro translation products that increased in abundance after GA3 treatment were detected.
番茄（Lycopersicon esculentum Mill.）的gib1突变体缺乏内源赤霉素，表现出包括极度矮化、发芽率降低和花发育异常等表型，而施加赤霉酸（GA3）可使其恢复正常。先前的研究表明，在gib1突变体的雄蕊中，花粉母细胞发育停滞在减数分裂前的G1期（Jacobsen和Olszewski，1991年，《植物生理学》97卷，409 - 414页）。用GA3处理发育停滞的花朵后，花粉母细胞发育恢复且同步。本研究检测了赤霉素诱导的gib1突变体发育停滞雄蕊和营养芽中可翻译mRNA群体的变化。在用GA3处理使发育停滞的雄蕊恢复发育后，我们分别持续检测到14种和20种体外翻译产物丰度的增加和减少。其中一些变化在处理后8小时首次检测到，因此代表了经GA3处理恢复发育的雄蕊中观察到的首批变化。在gib1营养芽中，GA3处理后6 - 24小时内，13种体外翻译产物的丰度下降。然而，未检测到GA3处理后丰度增加的体外翻译产物。
