Johansson K, Jönsson-Pettersson G, Gorton L, Marko-Varga G, Csöregi E
Department of Analytical Chemistry, University of Lund, Sweden.
J Biotechnol. 1993 Dec;31(3):301-16. doi: 10.1016/0168-1656(93)90076-y.
A reagentless carbon paste electrode chemically modified with covalently bound alcohol oxidase and horse-radish peroxidase was examined as a selective sensor in flow injection and column liquid chromatography. A combination of carbodiimide, glutaraldehyde, and polyethyleneimine was used for immobilizing the enzymes in the paste. The surface of the electrodes was protected by first forming a layer of electropolymerized ortho-phenylenediamine followed by deposition of a cation exchange membrane (Eastman AQ 29D). The electrodes were used for detection of hydrogen peroxide, methanol, ethanol, propanol, isopropanol, and butanol. Preliminary investigations of the use of this sensor for bioprocess control are reported.
