Park T G, Hoffman A S
School of Pharmacy, Temple University, Philadelphia, Pennsylvania 19140.
Biotechnol Prog. 1994 Jan-Feb;10(1):82-6. doi: 10.1021/bp00025a010.
The pore sizes of various temperature-sensitive hydrogel beads have been estimated at different temperatures by a gel filtration method using several probe molecules of known molecular weight. The hydrogel beads were prepared by inverse suspension copolymerizations of a series of N-isopropylacrylamide (NIPAAm) and acrylamide (AAm) monomers with a small amount of cross-linker. These hydrogel beads exhibit LCST (lower critical solution temperature) behavior in aqueous solution, that is, expanding and swelling when cooled below the LCST and shrinking and collapsing when heated above the LCST. The pore sizes of these temperature-sensitive hydrogel beads are affected significantly by both the temperature and the gel composition, but not much by cross-linker concentration.
通过使用几种已知分子量的探针分子的凝胶过滤方法，在不同温度下估算了各种温度敏感水凝胶珠的孔径。水凝胶珠是通过一系列N-异丙基丙烯酰胺（NIPAAm）和丙烯酰胺（AAm）单体与少量交联剂的反相悬浮共聚制备的。这些水凝胶珠在水溶液中表现出最低临界溶液温度（LCST）行为，即在冷却至低于LCST时膨胀和溶胀，在加热至高于LCST时收缩和塌陷。这些温度敏感水凝胶珠的孔径受温度和凝胶组成的显著影响，但受交联剂浓度的影响不大。
