Mellors A, Sutherland D R
Department of Chemistry and Biochemistry, University of Guelph, Ontario, Canada.
Trends Biotechnol. 1994 Jan;12(1):15-8. doi: 10.1016/0167-7799(94)90006-X.
There are a variety of enzymes available that are able to cleave glycoproteins, including enzymes that are specific for carbohydrate-carbohydrate linkages, carbohydrate-protein bonds and the peptide backbone. Such enzymes are useful for determining the sites of glycosylation within proteins, and for releasing glycan structures for subsequent carbohydrate analysis. One protease has been identified as being specific for O-sialoglycoproteins and can be used to identify such molecules and their epitope regions. The lack of cytotoxicity and the narrow specificity of this enzyme provides an improved method for the immunomagnetic selection of human bone-marrow stem-cells.
有多种酶可用于切割糖蛋白，包括对碳水化合物-碳水化合物连接、碳水化合物-蛋白质键和肽主链具有特异性的酶。这些酶可用于确定蛋白质内的糖基化位点，并释放聚糖结构以进行后续的碳水化合物分析。一种蛋白酶已被鉴定为对O-唾液酸糖蛋白具有特异性，可用于鉴定此类分子及其表位区域。这种酶缺乏细胞毒性且特异性狭窄，为人类骨髓干细胞的免疫磁选提供了一种改进方法。
