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新型生物分离技术中的蛋白质失活

Protein inactivations during novel bioseparation techniques.

作者信息

Sadana A

机构信息

Chemical Engineering Department, University of Mississippi, University 38677-9740.

出版信息

Bioseparation. 1994 Feb;4(1):39-61.

PMID:7764586
Abstract

An analysis is presented for the quantitative and qualitative inactivation of proteins and other bioproducts during their separation utilizing the reverse micellar and the aqueous two-phase extraction techniques. Information on the influence of different parameters on the quantitative yields of the bioproducts separated is available, and more information is being gathered to provide further physical insights into improving the quantitative yields. However, very little information is available on the qualitative nature of the bioproducts separated utilizing either the reverse micelle or the aqueous two-phase extraction technique. More information is definitely required on the qualitative nature of the bioproducts separated by the above techniques to assist in their proper evaluation as effective bioseparation techniques.

摘要

本文对利用反胶束和双水相萃取技术分离蛋白质及其他生物制品过程中的定量和定性失活进行了分析。关于不同参数对分离得到的生物制品定量产率的影响已有相关信息,并且正在收集更多信息以进一步深入了解提高定量产率的物理因素。然而,关于利用反胶束或双水相萃取技术分离得到的生物制品的定性特征,可用信息非常少。显然,需要更多关于通过上述技术分离得到的生物制品定性特征的信息,以帮助对其作为有效的生物分离技术进行恰当评估。

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