Sadana A
Chemical Engineering Department, University of Mississippi, University 38677-9740.
Bioseparation. 1992;3(2-3):145-65.
The denaturation of proteins and other biological macromolecules such as gentamycin, mRNAs, and long-chain fatty acids during their separation by different chromatographic techniques is analyzed. Non-conventional techniques such as centrifugal partition chromatography are also examined. Particular attention is paid to the denaturing mechanisms prevalent under processing conditions, and how denaturation may perhaps be alleviated under laboratory conditions or during scale-up. The available mechanistic studies shed physical insights into the conformational behavior of proteins on chromatographic columns. Mechanistic studies of other biological macromolecule separation on columns is rare. Numbers for both recovery and purity of the biological product are presented wherever available. Scale-up studies are rare, nevertheless, those that are presented together do provide significant and valuable information, and may be generalized to other systems with caution.
分析了蛋白质和其他生物大分子（如庆大霉素、信使核糖核酸和长链脂肪酸）在通过不同色谱技术分离过程中的变性情况。还研究了离心分配色谱等非常规技术。特别关注了加工条件下普遍存在的变性机制，以及在实验室条件下或放大过程中如何减轻变性。现有的机理研究为蛋白质在色谱柱上的构象行为提供了物理见解。关于其他生物大分子在柱上分离的机理研究很少。只要有数据，就会列出生物产品的回收率和纯度。放大研究很少，不过，那些一起呈现的放大研究确实提供了重要且有价值的信息，并且可以谨慎地推广到其他系统。
