通过添加积雪草提取物，在无血清培养基中增强成纤维细胞对微载体的附着及组织型纤溶酶原激活物的产生。
Enhancement of the attachment on microcarriers and tPA production by fibroblast cells in a serum-free medium by the addition of the extracts of Centella asiatica.
作者信息
Kim Y N, Park Y S, Kim H K, Jeon B C, Youn S E, Lee H Y
机构信息
Department of Food Engineering, Kangwon National University, Chunchon, Korea.
出版信息
Cytotechnology. 1993;13(3):221-6. doi: 10.1007/BF00749818.
The addition of ethanol extracts of Centella asiatica showed a remarkable enhancement of fibroblast cells attachment to Cytodex beads in serum-free (SF) medium. It also improves tPA production in both batch and perfusion cultivations. The optimal concentration for SF medium was determined as 2 ppm of the extracts when using Cytodex III. In batch cultivation a high specific tPA production rate was obtained, compared to that from 5% FBS containing medium. However, a fast specific growth rate was observed in 5% FBS medium. In perfusion cultivation a reasonably good cell density and tPA production was achieved at a perfusion rate of 2.4 x 10(6) (viable cell/ml) and 0.65 (micrograms/ml), respectively at 22 ml/min.
添加积雪草乙醇提取物后，在无血清（SF）培养基中，成纤维细胞与Cytodex微载体珠的附着能力显著增强。在分批培养和灌注培养中，它还能提高组织型纤溶酶原激活剂（tPA）的产量。使用Cytodex III时，SF培养基的最佳提取物浓度确定为2 ppm。在分批培养中，与含5%胎牛血清（FBS）的培养基相比，获得了较高的tPA比生产速率。然而，在5% FBS培养基中观察到较快的比生长速率。在灌注培养中，当灌注速率分别为22 ml/min、2.4×10⁶（活细胞/ml）和0.65（微克/ml）时，实现了相当良好的细胞密度和tPA产量。
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