Roitsch T, Tanner W
Institut für Zellbiologie und Pflanzenphysiologie, Universität Regensburg, Germany.
Planta. 1994;193(3):365-71. doi: 10.1007/BF00201814.
Photoautotrophic suspension-culture cells of Chenopodium rubrum L. were shifted to mixotrophic growth by adding glucose to investigate whether the activities of plant sugar transporters, as well as the expression of the corresponding genes, are regulated in response to sugars. The rate of D-glucose uptake was shown not to be affected by mixotrophic growth in the presence of D-glucose. The polymerase chain reaction (PCR) technique was applied to amplify cDNA and genomic fragments from monosaccharide-carrier genes. Seven members of a monosaccharide-carrier family were identified of which three were found to be expressed in the suspension-culture cells. The expression of the monosaccharide-carrier genes was independent of the presence of D-glucose.
通过添加葡萄糖将藜的光合自养悬浮培养细胞转变为兼养生长，以研究植物糖转运蛋白的活性以及相应基因的表达是否会响应糖类而受到调节。在存在D-葡萄糖的情况下，兼养生长并未显示出对D-葡萄糖摄取速率有影响。应用聚合酶链反应（PCR）技术从单糖载体基因中扩增cDNA和基因组片段。鉴定出一个单糖载体家族的七个成员，其中三个在悬浮培养细胞中表达。单糖载体基因的表达与D-葡萄糖的存在无关。
