Sander C
European Molecular Biology Laboratory, Heidelberg, Germany.
Trends Biotechnol. 1994 May;12(5):163-7. doi: 10.1016/0167-7799(94)90077-9.
The design of proteins or peptides with novel functions can be achieved either by modifying existing molecules or by inventing entirely new structures and sequences that are unknown in nature. Combinatorial-design strategies have led to the first de novo proteins, but these still lack some of the desired attributes. The most promising practical strategies for developing proteins with useful biological or chemical function combine theoretical design with experimental screening or selection systems.
具有新功能的蛋白质或肽的设计可以通过修饰现有分子来实现，也可以通过创造自然界中未知的全新结构和序列来实现。组合设计策略已经催生了首批从头设计的蛋白质，但这些蛋白质仍缺乏一些理想的特性。开发具有有用生物或化学功能的蛋白质最有前景的实用策略是将理论设计与实验筛选或选择系统相结合。
