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与油菜自交不亲和性相关的S-糖蛋白序列及其在十字花科中的分布。

The sequences of S-glycoproteins involved in self-incompatibility of Brassica campestris and their distribution among Brassicaceae.

作者信息

Yamakawa S, Shiba H, Watanabe M, Shiozawa H, Takayama S, Hinata K, Isogai A, Suzuki A

机构信息

Department of Agricultural Chemistry, University of Tokyo, Japan.

出版信息

Biosci Biotechnol Biochem. 1994 May;58(5):921-5. doi: 10.1271/bbb.58.921.

Abstract

S-Glycoproteins have been thought to play a role in the self-incompatibility in Brassica plants. Two cDNAs encoding S-glycoproteins were isolated from stigmas of two strains of Brassica campestris. Their amino acid sequences are highly homologous to each other. However, the numbers of the potential glycosylation sites are different, although most of them are conserved. In the middle part of the sequences, there are two supervariable regions which might contribute to the specificity of each S-glycoprotein. Genomic Southern analysis showed many bands hybridizing with cDNA of S-glycoproteins in various Brassica species including self-compatible species.

摘要

S糖蛋白被认为在芸苔属植物的自交不亲和性中起作用。从两种油菜菌株的柱头中分离出两个编码S糖蛋白的cDNA。它们的氨基酸序列彼此高度同源。然而,潜在糖基化位点的数量不同,尽管其中大多数是保守的。在序列的中部,有两个超可变区,这可能有助于每种S糖蛋白的特异性。基因组Southern分析显示,在包括自交亲和物种在内的各种芸苔属物种中,有许多条带与S糖蛋白的cDNA杂交。

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