Robert L S, Allard S, Franklin T M, Trick M
Plant Research Centre, Central Experimental Farm, Ottawa, Ontario, Canada.
Mol Gen Genet. 1994 Jan;242(2):209-16. doi: 10.1007/BF00391015.
Brassica napus is an amphidiploid plant which is self-compatible even though it is derived from hybridisation of the self-incompatible species B. oleracea and B. campestris. Experiments were undertaken to establish if S-locus glycoprotein (SLG) genes exist in B. napus and whether these are expressed as in self-incompatible Brassica species. Two different stigma-specific cDNA sequences homologous to SLG genes were obtained from the B. napus cultivar Westar. One of these sequences, SLGWS1, displayed highest homology to class I SLG alleles, whereas the other, SLGWS2, showed greatest homology to class II SLG genes. Both were expressed at high levels in Westar stigmas following a developmental pattern typical of SLG genes in the self-incompatible diploids. We infer that they represent the endogenous SLG genes at the two homologous S-loci. The occurrence of normally expressed SLG genes and its relevance to the self-compatible phenotype of B. napus is discussed.
甘蓝型油菜是一种异源四倍体植物，尽管它源自自交不亲和物种甘蓝和白菜的杂交，但它自身是可育的。开展了实验来确定甘蓝型油菜中是否存在S位点糖蛋白（SLG）基因，以及这些基因是否如在自交不亲和的芸苔属物种中那样表达。从甘蓝型油菜品种Westar中获得了两个与SLG基因同源的不同的柱头特异性cDNA序列。其中一个序列SLGWS1与I类SLG等位基因具有最高的同源性，而另一个序列SLGWS2与II类SLG基因具有最大的同源性。在自交不亲和二倍体中，这两个序列都按照SLG基因典型的发育模式在Westar柱头中高水平表达。我们推断它们代表了两个同源S位点上的内源性SLG基因。本文讨论了正常表达的SLG基因的存在及其与甘蓝型油菜自交亲和表型的相关性。
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