酵母人工染色体、细菌人工染色体、P1人工染色体和哺乳动物人工染色体：作为研究工具的人工染色体
YACs, BACs, PACs and MACs: artificial chromosomes as research tools.
作者信息
Monaco A P, Larin Z
机构信息
Imperial Cancer Research Fund Laboratories, Institute of Molecular Medicine, John Radcliffe Hospital, Headington, Oxford, UK.
出版信息
Trends Biotechnol. 1994 Jul;12(7):280-6. doi: 10.1016/0167-7799(94)90140-6.
PMID:7765076
Abstract
Yeast artificial chromosomes (YACs) have become essential research tools as they enable large fragments of DNA to be cloned. In order to overcome several disadvantages of YACs, including chimaerism and instability, several complementary bacterial artificial chromosome (BAC) vectors have been developed. More recently, attempts are being made to construct artificial chromosomes in mammalian cells (MACs).
摘要
酵母人工染色体（YACs）已成为重要的研究工具，因为它们能够克隆大片段DNA。为了克服YACs的几个缺点，包括嵌合现象和不稳定性，人们开发了几种互补的细菌人工染色体（BAC）载体。最近，人们正在尝试在哺乳动物细胞中构建人工染色体（MACs）。
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