Kawahara H, Mitsuda S, Kumazawa E, Takeshita Y
Snow Brand Milk Products Co., Ltd., Tochigi, Japan.
Cytotechnology. 1994;14(1):61-6. doi: 10.1007/BF00772196.
A novel bioreactor system developed for high-density cultures of suspended mammalian cells is described using a tangential-flow filtration device outside the culture vessel to separate viable cells from spent medium. The filtration device is based on thin porous microfiltration membranes with a pore size of 0.20-0.65 microns. Because cells have a diameter of about 10-20 microns, they cannot permeate these membranes with the spent medium. So, allowing a perfusion culture to be created using this system. In most membrane filtration systems, clogging of the membranes has made long-term operation difficult. In this system, however, high pressure is not applied directly to the membrane, thus minimizing clogging. Also, clogging of the membrane was prevented by washing the membrane surface once a day, and increasing the membrane surface area. With this system, FM-3A cells were cultured and maintained at a high density of 3.0 x 10(7) cells/ml for two weeks, and a continuous culture was supported for as long as 34 days.
本文描述了一种新型生物反应器系统，该系统用于悬浮哺乳动物细胞的高密度培养，它在培养容器外部使用切向流过滤装置将活细胞与用过的培养基分离。该过滤装置基于孔径为0.20 - 0.65微米的薄多孔微滤膜。由于细胞直径约为10 - 20微米，它们不能与用过的培养基一起透过这些膜。因此，使用该系统可以创建灌注培养。在大多数膜过滤系统中，膜的堵塞使得长期操作变得困难。然而，在该系统中，不会直接对膜施加高压，从而将堵塞降至最低。此外，通过每天清洗膜表面并增加膜表面积来防止膜的堵塞。使用该系统，FM - 3A细胞以3.0×10⁷个细胞/毫升的高密度培养并维持了两周，并且支持连续培养长达34天。
