Seewöster T, Wilmsmann S, Werner A, Lehmann J
Institute of Cell Culture Technology, Faculty of Technical Sciences, University of Bielefeld, Germany.
Ann N Y Acad Sci. 1997 Dec 31;831:244-8.
Based on the laminar structure of the human liver tissue, a high cell density membrane bioreactor was developed that emulates a cell layer thickness of 40 microns. The "biochip" consists of a platinum-coated metal cell grid covered with two microfiltration membranes to form separate cell chambers of defined volume. Starting with a continuous chemostat process, the viability of a model suspension cell culture could be stabilized at 98%. In a second step these cells were transferred into the biochip system and were cultivated successfully for several days under tissue-like cell densities in a modified membrane holder under cross-flow conditions.
基于人类肝脏组织的层状结构，开发了一种高细胞密度膜生物反应器，该反应器模拟了40微米的细胞层厚度。这种“生物芯片”由一个涂有铂的金属细胞网格组成，上面覆盖着两个微滤膜，形成了特定体积的独立细胞室。从连续恒化器过程开始，模型悬浮细胞培养物的活力可以稳定在98%。第二步，将这些细胞转移到生物芯片系统中，并在错流条件下的改良膜支架中，以类似组织的细胞密度成功培养了几天。
