Pol A, Op den Camp H J, Mees S G, Kersten M A, van der Drift C
Department of Microbiology & Evolutionary Biology, Faculty of Science, University of Nijmegen, The Netherlands.
Biodegradation. 1994 Jun;5(2):105-12. doi: 10.1007/BF00700635.
The methylotrophic bacterium Hyphomicrobium VS was enriched and isolated, using activated sewage sludge as inoculum in mineral medium containing dimethylsulfide (DMS) at a low concentration to prevent toxicity. DMS concentrations above 1 mM proved to be growth inhibiting. Hyphomicrobium VS could use DMS, dimethylsulfoxide (DMSO), methanol, formaldehyde, formate, and methylated amines as carbon and energy source. Carbon was assimilated via the serine pathway. DMS-grown cells respired sulfide, thiosulfate, methanethiol, dimethyldisulfide and dimethyltrisulfide. To test Hyphomicrobium VS for application in biofiltration of air polluted with volatile sulfur compounds two laboratory scale trickling biofilters with polyurethane and lava stone as carrier material were started up by inoculation with this bacterium. Both methanol- and DMS-grown cells could be used. Only a short adaptation period was needed. Short term experiments showed that high concentrations of DMS (1-2 mumol l-1) were removed very efficiently by the biofilters at space velocities up to 100 h-1.
以活性污水污泥为接种物，在含有低浓度二甲基硫醚（DMS）的矿物培养基中富集并分离出甲基营养型细菌Hyphomicrobium VS，以防止毒性。事实证明，DMS浓度高于1 mM会抑制生长。Hyphomicrobium VS可以利用DMS、二甲基亚砜（DMSO）、甲醇、甲醛、甲酸和甲基化胺作为碳源和能源。碳通过丝氨酸途径被同化。以DMS为生长底物的细胞能以硫化物、硫代硫酸盐、甲硫醇、二甲基二硫醚和二甲基三硫醚作为呼吸底物。为了测试Hyphomicrobium VS在生物过滤挥发性硫化合物污染空气方面的应用，用该细菌接种启动了两个以聚氨酯和火山岩为载体材料的实验室规模滴滤式生物滤池。以甲醇和DMS为生长底物的细胞均可使用。只需较短的适应期。短期实验表明，在空速高达100 h-1时，生物滤池能非常有效地去除高浓度的DMS（1-2 μmol l-1）。
