Rost B, Sander C
European Molecular Biology Laboratory, Heidelberg, Germany.
Curr Opin Biotechnol. 1994 Aug;5(4):372-80. doi: 10.1016/0958-1669(94)90045-0.
Although the 'structure from sequence' prediction problem remains fundamentally unsolved, new and promising methods in one, two and three dimensions have reopened the field. Significantly improved one-dimensional prediction of secondary structure from multiple sequence alignments is now in routine use. In the two-dimensional approach, inter-residue contacts can be detected by analysis of correlated mutations, albeit with low accuracy. Finally, three-dimensional methods, in which pseudopotentials or information values are derived from the databases, are proving their value for distinguishing between correct and incorrect models.
尽管“从序列预测结构”这一问题从根本上来说仍未解决，但一维、二维和三维领域出现的新的、有前景的方法为该领域带来了新的生机。目前，通过多序列比对对二级结构进行的一维预测有了显著改进，并已得到常规应用。在二维方法中，尽管准确性较低，但可以通过分析相关突变来检测残基间的相互作用。最后，三维方法通过从数据库中导出伪势或信息值，在区分正确和错误模型方面证明了其价值。
