Minai Y, Hisatsune T, Nishijima K, Enomoto A, Kaminogawa S
Department of Agricultural Chemistry, University of Tokyo, Japan.
Cytotechnology. 1994;14(2):81-7. doi: 10.1007/BF00758172.
In a previous study, we established CD8+ suppressor T cell (Ts) clone 13G2 which produced the suppressive lymphokine, interleukin-10 (IL-10). In this study, we examined what physiological activator could induce both production of IL-10 from 13G2 and the proliferation of 13G2. Both the antigenic stimulation mimicked by the anti-CD3 antibody and the T cell growth factor interleukin-2 (IL-2) induced IL-10 production from the 13G2 clone equally well. 13G2 cells proliferated remarkably with IL-2 stimulation, while anti-CD3 only slightly induced proliferation of the clone. 13G2 cells also produced IL-10 in the presence of hydroxyurea which blocked transit of cells from G1 to S phase. However, cycloheximide blocked the production of IL-10 from the Ts clone. The study demonstrates that both the anti-CD3 antibody and IL-2 induced IL-10 synthesis of the Ts clone equally well, and the proliferative response of Ts cells was induced more by IL-2 than by anti-CD3. IL-2 proved to be a good stimulator for Ts cells to produce suppressive lymphokine and to multiply their population.
在先前的一项研究中，我们建立了产生抑制性淋巴因子白细胞介素-10（IL-10）的CD8 +抑制性T细胞（Ts）克隆13G2。在本研究中，我们研究了何种生理激活剂能够诱导13G2产生IL-10以及13G2的增殖。抗CD3抗体模拟的抗原刺激和T细胞生长因子白细胞介素-2（IL-2）诱导13G2克隆产生IL-10的效果同样良好。13G2细胞在IL-2刺激下显著增殖，而抗CD3仅轻微诱导该克隆的增殖。13G2细胞在存在羟基脲的情况下也产生IL-10，羟基脲可阻断细胞从G1期到S期的过渡。然而，放线菌酮可阻断Ts克隆产生IL-10。该研究表明，抗CD3抗体和IL-2诱导Ts克隆合成IL-10的效果同样良好，并且Ts细胞的增殖反应由IL-2诱导的程度比抗CD3更高。事实证明，IL-2是Ts细胞产生抑制性淋巴因子并使其数量增加的良好刺激剂。
