Aono R, Negishi T, Aibe K, Inoue A, Horikoshi K
Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
Biosci Biotechnol Biochem. 1994 Jul;58(7):1231-5. doi: 10.1271/bbb.58.1231.
The extent of organic solvent tolerance was variable among strains of Escherichia coli K-12. Genetic analyses of n-hexane-tolerant strains indicated that a number of genes were involved in the solvent-tolerance phenotype. One such gene, designated ostA, was mapped at 1.2 min, close to pdxA. Transduction of ostA from a n-hexane-sensitive strain to a n-hexane-tolerant strain generated n-hexane-sensitive transductants. The sensitive transductant restored n-hexane-tolerance by transduction of ostA from a tolerant strain. Thus, the gene ostA is one of the genes that contributes to deciding the level of organic solvent tolerance in E. coli.
大肠杆菌K-12菌株对有机溶剂的耐受程度各不相同。对耐正己烷菌株的遗传分析表明，许多基因与耐溶剂表型有关。其中一个这样的基因，命名为ostA，定位于1.2分钟处，靠近pdxA。将ostA从正己烷敏感菌株转导到正己烷耐受菌株中产生了正己烷敏感的转导子。通过从耐受菌株中转导ostA，敏感转导子恢复了正己烷耐受性。因此，ostA基因是决定大肠杆菌有机溶剂耐受水平的基因之一。
