Aono R, Negishi T, Nakajima H
Department of Bioengineering, Faculty of Bioscience and Biotechnology, Tokyo Institute of Technology, Yokohama, Japan.
Appl Environ Microbiol. 1994 Dec;60(12):4624-6. doi: 10.1128/aem.60.12.4624-4626.1994.
A variety of genes are involved in determining the level of organic solvent tolerance of Escherichia coli K-12. Gene ostA is one of the genes contributing to the level of organic solvent tolerance. This gene was cloned from an n-hexane-tolerant strain of E. coli, JA300. A JA300-based n-hexane-sensitive strain, OST4251, was converted to the n-hexane-tolerant phenotype by transformation with DNA containing the ostA gene derived from JA300. Thus, the cloned ostA gene complemented the n-hexane-sensitive phenotype of OST4251.
多种基因参与决定大肠杆菌K-12对有机溶剂的耐受水平。基因ostA是影响有机溶剂耐受水平的基因之一。该基因是从耐正己烷的大肠杆菌菌株JA300中克隆得到的。通过用含有源自JA300的ostA基因的DNA进行转化，将基于JA300的正己烷敏感菌株OST4251转变为耐正己烷表型。因此，克隆的ostA基因弥补了OST4251的正己烷敏感表型。
