Hidaka Y, Hasegawa M, Nakahara T, Hoshino T
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
Biosci Biotechnol Biochem. 1994 Jul;58(7):1338-9. doi: 10.1271/bbb.58.1338.
Thermus thermophilus mutants carrying unlinked double auxotrophic markers were transformed with wild type chromosomal DNA at various growth phases. The percentages of competent cells in the total population were calculated based on the results of transformation efficiencies for single or double markers. It was concluded that all the T. thermophilus cells at any growth phase were competent.
携带不连锁双营养缺陷型标记的嗜热栖热菌突变体在不同生长阶段用野生型染色体DNA进行转化。根据单标记或双标记转化效率的结果计算总群体中感受态细胞的百分比。得出的结论是，嗜热栖热菌在任何生长阶段的所有细胞都是感受态的。
