Bassam B J, Bentley S
Cooperative Research Centre for Tropical Plant Pathology, University of Queensland, St Lucia, Australia.
Australas Biotechnol. 1994 Jul-Aug;4(4):232-6.
A recent variation of the PCR has allowed DNA fingerprints to be obtained independently of prior sequence information and with unparalleled ease. Several approaches, conveniently grouped under the general term of arbitrary primer technology (APT), include the popular RAPD, DAF, and AP-PCR methods. A great deal of attention has been focussed on these methods and questions have arisen regarding reproducibility, DNA fingerprint resolution, and even the future of the technology itself. Here we discuss these issues and examine some of the unique properties of DNA amplification using a single short arbitrary primer.
聚合酶链式反应（PCR）的最新变体使得无需预先的序列信息就能极其轻松地获得DNA指纹图谱。几种方法，方便地归类于通用术语“任意引物技术（APT）”之下，包括广为人知的随机扩增多态性DNA（RAPD）、DNA扩增指纹分析（DAF）和任意引物PCR（AP-PCR）方法。这些方法受到了极大的关注，关于其可重复性、DNA指纹分辨率甚至该技术本身的未来都出现了一些问题。在此，我们将讨论这些问题，并研究使用单个短任意引物进行DNA扩增的一些独特特性。
