Matsuura S, Kita H, Takagaki Y
Division of Biochemistry, Iatron Laboratories, Inc., Chiba, Japan.
Biosci Biotechnol Biochem. 1994 Aug;58(8):1471-5. doi: 10.1271/bbb.58.1471.
The specificity of five mouse monoclonal antibodies to okadaic acid was studied for use in an enzyme-linked immunosorbent assay of okadaic acid and its analogs. OA8-2 and OA22-22 antibodies (IgG2a-kappa), which bind more strongly to dinophysistoxin-1 and 7-O-palmitoyl-dinophysistoxin-1 than to okaic acid or 7-O-palmitoyl-okadaic acid in 50% aqueous methanol, were useful in the detection of dinophysistoxins-1 and -3. OA10-8 (IgG1-kappa), which binds more strongly to 7-O-palmitoyl-okadaic acid and 7-O-palmitoyl-dinophysistoxin-1 than to okadaic or dinophysistoxin-1 in 50% aqueous methanol, was useful in the detection of dinophysistoxin-3. OA423-3 (IgG1-kappa), which binds weakly to dinophysistoxin-1 and 7-O-palmitoyl-dinophysistoxin-1 in 20% aqueous methanol, was useful in the selective detection of okadaic acid. OA958-2 (IgG1-kappa), which binds with equal strength to each of the four toxins in methanol, was useful in the detection of all okadaic acid analogs, and the minimum detectable concentration was 30 ng/ml. OA423-3 and OA958-2 retained their binding ability in 50% acetone, ethyl ether, or benzene in methanol.
研究了五种抗冈田酸小鼠单克隆抗体在冈田酸及其类似物酶联免疫吸附测定中的特异性。OA8 - 2和OA22 - 22抗体（IgG2a - κ）在50%甲醇水溶液中对鳍藻毒素 - 1和7 - O - 棕榈酰鳍藻毒素 - 1的结合力比对冈田酸或7 - O - 棕榈酰冈田酸更强，可用于检测鳍藻毒素 - 1和 - 3。OA10 - 8（IgG1 - κ）在50%甲醇水溶液中对7 - O - 棕榈酰冈田酸和7 - O - 棕榈酰鳍藻毒素 - 1的结合力比对冈田酸或鳍藻毒素 - 1更强，可用于检测鳍藻毒素 - 3。OA423 - 3（IgG1 - κ）在20%甲醇水溶液中对鳍藻毒素 - 1和7 - O - 棕榈酰鳍藻毒素 - 1的结合较弱，可用于选择性检测冈田酸。OA958 - 2（IgG1 - κ）在甲醇中对四种毒素的结合力相同，可用于检测所有冈田酸类似物，最低可检测浓度为30 ng/ml。OA423 - 3和OA958 - 2在甲醇与50%丙酮、乙醚或苯的混合溶液中仍保持其结合能力。
