一种抗冈田酸抗体对鳍藻毒素-4（DTX-4）、鳍藻毒素-5（DTX-5）和冈田酸二醇酯的交叉反应性。

Cross-reactivity of an anti-okadaic acid antibody to dinophysistoxin-4 (DTX-4), dinophysistoxin-5 (DTX-5), and an okadaic acid diol ester.

作者信息

Lawrence J E, Cembella A D, Ross N W, Wright J L

机构信息

Department of Oceanography, Dalhousie University, Halifax, NS, Canada.

出版信息

Toxicon. 1998 Aug;36(8):1193-6. doi: 10.1016/s0041-0101(98)00005-1.

DOI:10.1016/s0041-0101(98)00005-1

PMID:9690786

Abstract

The cross-reactivity of the 6/50 monoclonal anti-okadaic acid antibody (mAb) to the recently discovered diarrhetic shellfish poisoning (DSP) metabolites dinophysistoxin-4 (DTX-4), dinophysistoxin-5 (DTX-5), and an okadaic acid (OA) diol ester was determined using a competitive indirect enzyme-linked immunosorbent assay (ELISA). The reactivity of the antibody to these molecules was compared to that with OA; the 6/50 mAb recognized all of these DSP compounds with equal sensitivity within the working range of the antibody (10-100 nM for OA). This confirms the ability of the antibody to detect all DSP compounds when used in analyses including ELISA and immunocytochemistry.

摘要

使用竞争性间接酶联免疫吸附测定（ELISA）法，测定了6/50抗冈田酸单克隆抗体（mAb）与最近发现的腹泻性贝类毒素（DSP）代谢产物鳍藻毒素-4（DTX-4）、鳍藻毒素-5（DTX-5）以及一种冈田酸（OA）二醇酯的交叉反应性。将该抗体对这些分子的反应性与对OA的反应性进行了比较；在抗体的工作范围内（OA为10 - 100 nM），6/50 mAb以相同的灵敏度识别所有这些DSP化合物。这证实了该抗体在用于包括ELISA和免疫细胞化学在内的分析时，能够检测所有DSP化合物。