Matsushita N, Oda H, Kobayashi K, Akaike T, Yoshida A
Laboratory of Nutritional Biochemistry, School of Agricultural Sciences, Nagoya University, Japan.
Biosci Biotechnol Biochem. 1994 Aug;58(8):1514-6. doi: 10.1271/bbb.58.1514.
Freshly isolated hepatocytes were cultured on an EHS-gel prepared from EHS-tumor, poly-N-p-vinylbenzyl-D-lactonamide (PVLA), and type I collagen (TIC). Hepatocytes on EHS-gel showed a spherical shape and much more strongly maintained the inducible expression of cytochrome P-450 genes which were lost on PVLA and TIC. Further, the expression of liver-specific genes were maintained on EHS gel at the highest level, and then higher on PVLA than TIC.
将刚分离出的肝细胞培养在由EHS肿瘤、聚-N-对乙烯基苄基-D-内酰胺（PVLA）和I型胶原蛋白（TIC）制备的EHS凝胶上。在EHS凝胶上的肝细胞呈球形，并且更强有力地维持了细胞色素P-450基因的诱导表达，而这些基因在PVLA和TIC上会丢失。此外，肝脏特异性基因的表达在EHS凝胶上维持在最高水平，然后在PVLA上高于在TIC上。
