嗜肺军团菌的随机扩增多态性DNA（RAPD）分析
Random amplified polymorphic DNA (RAPD) profiling of Legionella pneumophila.
作者信息
Sandery M, Coble J, McKersie-Donnolley S
机构信息
Microbiology Laboratory, New South Wales Health Department, Lidcombe, Australia.
出版信息
Lett Appl Microbiol. 1994 Oct;19(4):184-7. doi: 10.1111/j.1472-765x.1994.tb00939.x.
DNA (RAPD) profiling of Legionella pneumophila by PCR can be used to provide a simple and efficient comparison of clinical and environmental isolates. RAPD profiling is quicker and cheaper to perform than restriction fragment length polymorphism (RFLP) typing, eliminating the need for blotting, hybridization and detection. For some isolates, RAPD profiling is more discriminatory than RFLP typing, being able to distinguish between isolates with identical RFLP types.
通过聚合酶链反应（PCR）对嗜肺军团菌进行DNA（随机扩增多态性DNA，RAPD）分析，可用于对临床分离株和环境分离株进行简单有效的比较。与限制性片段长度多态性（RFLP）分型相比，RAPD分析操作更快且成本更低，无需进行印迹、杂交和检测。对于某些分离株，RAPD分析比RFLP分型更具鉴别力，能够区分具有相同RFLP类型的分离株。
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