用于检测蜡样芽孢杆菌腹泻性肠毒素的Tecra VIA试剂盒、Oxoid BCET-RPLA试剂盒和CHO细胞培养检测法的比较
Comparison of the Tecra VIA kit, Oxoid BCET-RPLA kit and CHO cell culture assay for the detection of Bacillus cereus diarrhoeal enterotoxin.
作者信息
Buchanan R L, Schultz F J
机构信息
US Department of Agriculture, ARS Eastern Regional Research Center, Philadelphia, PA 19118.
出版信息
Lett Appl Microbiol. 1994 Nov;19(5):353-6. doi: 10.1111/j.1472-765x.1994.tb00473.x.
Two commercial serological kits (Oxoid BCET-RPLA and Tecra VIA) and a Chinese hamster ovary (CHO) cell cytotonicity assay for the detection of Bacillus cereus diarrhoeal enterotoxin were compared. Eleven B. cereus strains and one enterotoxigenic B. thuringiensis strain were evaluated. Both kits and the CHO cell assay yielded positive toxin responses for cell-free culture filtrates from eight out of 11 diarrhoeal enterotoxigenic strains. An emetic enterotoxin producing strain was negative with all three assays. Two B. cereus strains were negative using the BCET-RPLA kit, but positive with the Tecra VIA kit and CHO cell assay. The BCET-RPLA indicated significant levels of enterotoxin after samples were boiled, whereas the CHO cell and Tecra assays were negative. Overall, the cell culture assay was the most sensitive. However, the Tecra VIA kit provided similar results and was better suited for the rapid detection of B. cereus diarrhoeal enterotoxin.
比较了两种用于检测蜡样芽孢杆菌腹泻性肠毒素的商业血清学试剂盒（Oxoid BCET-RPLA和Tecra VIA）以及一种中国仓鼠卵巢（CHO）细胞细胞毒性试验。评估了11株蜡样芽孢杆菌菌株和1株产肠毒素苏云金芽孢杆菌菌株。两种试剂盒和CHO细胞试验对11株腹泻性产肠毒素菌株中8株的无细胞培养滤液均产生了阳性毒素反应。一株产催吐性肠毒素的菌株在所有三种试验中均为阴性。使用BCET-RPLA试剂盒时，有两株蜡样芽孢杆菌菌株为阴性，但使用Tecra VIA试剂盒和CHO细胞试验时为阳性。BCET-RPLA显示样品煮沸后肠毒素水平显著，而CHO细胞试验和Tecra试验为阴性。总体而言，细胞培养试验最敏感。然而，Tecra VIA试剂盒提供了相似的结果，更适合快速检测蜡样芽孢杆菌腹泻性肠毒素。
Zotero 插件