Day T L, Tatani S R, Notermans S, Bennett R W
Department of Food Science and Nutrition, University of Minnesota, St Paul 55108.
J Appl Bacteriol. 1994 Jul;77(1):9-13. doi: 10.1111/j.1365-2672.1994.tb03037.x.
Fourteen strains of Bacillus cereus isolated from different sources were examined for their ability to produce diarrhoeal enterotoxin by two commercial immunoassay kits (Oxoid BCET-RPLA and Tecra ELISA) and the microslide immunodiffusion assay. One strain that was positive in monkey feedings, as well as a number of other strains isolated from diarrhoeal outbreaks, gave positive results in the ELISA and negative results in the RPLA test systems. When tested in the microslide assay, these strains produced only one antigen which formed a line of identity with the reference toxin. The results of the control toxins provided with the kits substantiated that the two commercial assays did not detect the same antigen. Cultures positive with both assay kits were shown to produce diarrhoeal enterotoxin (by a line of identity) and other antigens in the microslide immunodiffusion assay.
使用两种商用免疫测定试剂盒（Oxoid BCET-RPLA和Tecra ELISA）以及微量玻片免疫扩散试验，对从不同来源分离出的14株蜡样芽孢杆菌产生腹泻性肠毒素的能力进行了检测。一株在猴喂养试验中呈阳性的菌株，以及从腹泻暴发中分离出的其他一些菌株，在ELISA检测中呈阳性，而在RPLA检测系统中呈阴性。在微量玻片试验中检测时，这些菌株仅产生一种抗原，该抗原与参考毒素形成一条同一线。试剂盒所提供的对照毒素的检测结果证实，这两种商用检测方法检测的不是同一种抗原。在微量玻片免疫扩散试验中，两种检测试剂盒均呈阳性的培养物显示产生腹泻性肠毒素（通过一条同一线）和其他抗原。
