用与Eudragit S - 100相连的大豆胰蛋白酶抑制剂进行胰蛋白酶的亲和沉淀。

Affinity precipitation of trypsin with soybean trypsin inhibitor linked Eudragit S-100.

作者信息

Kumar A, Gupta M N

机构信息

Chemistry Department, Indian Institute of Technology, New Delhi.

出版信息

J Biotechnol. 1994 Sep 30;37(2):185-9. doi: 10.1016/0168-1656(94)90010-8.

DOI:10.1016/0168-1656(94)90010-8

PMID:7765456

Abstract

Soybean trypsin inhibitor linked to Eudragit S-100 was used for the affinity precipitation of trypsin. Polymer and ligand concentrations used in conjugate preparation showed remarkable effect on the trypsin recovery. Trypsin precipitation efficiency amounted to 89% and recovery was 74%. The final purification of relatively crude commercial trypsin resulted in 1.85-fold purification. The SDS-PAGE analysis indicated significant purification. The precipitated enzyme activity was around 96% and recovered enzyme activity was 83%.

摘要

与Eudragit S - 100相连的大豆胰蛋白酶抑制剂用于胰蛋白酶的亲和沉淀。偶联物制备中使用的聚合物和配体浓度对胰蛋白酶回收率有显著影响。胰蛋白酶沉淀效率达89%，回收率为74%。相对粗制的商业胰蛋白酶经过最终纯化后得到了1.85倍的纯化效果。SDS - PAGE分析表明有显著的纯化效果。沉淀酶活性约为96%，回收酶活性为83%。