亲和沉淀过程中对Eudragit S-100非特异性吸附的评估。
An assessment of nonspecific adsorption to Eudragit S-100 during affinity precipitation.
作者信息
Kumar A, Gupta M N
机构信息
Chemistry Department, Indian Institute of Technology, Delhi, India.
出版信息
Mol Biotechnol. 1996 Aug;6(1):1-6. doi: 10.1007/BF02762318.
The problem of nonspecific adsorption to the reversibly soluble-insoluble polymers is of considerable importance in the design of an affinity precipitation protocol. It was seen that activation and coupling of the affinity ligand to the polymer changes the nature of the polymer surface in a significant fashion. The results with pure trypsin, partially purified trypsin preparation, and crude protein extract-containing protein inhibitors of trypsin and alpha-amylase and the reversibly soluble-insoluble polymer Eudragit S-100, show that nonspecific adsorption may not be a severe limitation in such systems.
在亲和沉淀方案的设计中，与可逆性可溶 - 不溶性聚合物的非特异性吸附问题相当重要。可以看出，亲和配体与聚合物的活化和偶联以显著方式改变了聚合物表面的性质。使用纯胰蛋白酶、部分纯化的胰蛋白酶制剂以及含有胰蛋白酶和α - 淀粉酶的蛋白质抑制剂的粗蛋白提取物与可逆性可溶 - 不溶性聚合物Eudragit S - 100的实验结果表明，在这类体系中，非特异性吸附可能并非严重的限制因素。
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