Kawarasaki Y, Nakano H, Yamane T
Department of Applied Biological Sciences, School of Agriculture, Nagoya University, Japan.
Biosci Biotechnol Biochem. 1994 Oct;58(10):1911-3. doi: 10.1271/bbb.58.1911.
Reaction conditions of cell-free protein synthesis using wheat germ extract were examined to prolong the period of protein synthesis in a batch reaction. By optimizing conditions for ATP regeneration system involved in the cell-free system, protein synthesis continued about 4 hours, so that about 17 micrograms dihydrofolate reductase protein was obtained in 1 ml of a reaction mixture. It suggests that maintaining ATP concentration is the primary requirement for long-life cell-free protein synthesis.
为了延长分批反应中蛋白质合成的时间，对使用小麦胚提取物进行无细胞蛋白质合成的反应条件进行了研究。通过优化无细胞系统中涉及的ATP再生系统的条件，蛋白质合成持续了约4小时，从而在1毫升反应混合物中获得了约17微克二氢叶酸还原酶蛋白。这表明维持ATP浓度是无细胞蛋白质长寿命合成的首要条件。
