Liu S C, Liu Y X, Webster D A, Stark B C
Department of Chemical and Biological Sciences, Illinois Institute of Technology, Chicago 60616.
Appl Microbiol Biotechnol. 1994 Nov;42(2-3):304-8. doi: 10.1007/BF00902733.
The 1668 base pairs (bp) downstream of the Vitreoscilla hemoglobin gene were sequenced in the hope of finding related genes that might be part of an operon. Instead, a sequence was found that constituted an open reading frame (ORF) of 569 amino acids (apparently the carboxy-terminal part of a larger ORF), in the direction opposite to the hemoglobin gene. This sequence was found to have 64% similarity with the 1685 bp at the 3' end of the Escherichia coli uvrA gene. The inferred amino acid sequence of the Vitreoscilla DNA has 69% similarity with the corresponding sequence of the E. coli uvrA protein, with similarities of 90, 100, and 85% in the helix-turn-helix, C-terminal ATP binding, and C-terminal zinc finger domains, respectively. The distance between the 3' ends of the Vitreoscilla hemoglobin and uvrA genes is 63 bp.
对透明颤菌血红蛋白基因下游的1668个碱基对（bp）进行了测序，以期找到可能作为操纵子一部分的相关基因。相反，发现了一个序列，它构成了一个由569个氨基酸组成的开放阅读框（ORF）（显然是一个更大ORF的羧基末端部分），其方向与血红蛋白基因相反。发现该序列与大肠杆菌uvrA基因3'端的1685 bp具有64%的相似性。透明颤菌DNA的推断氨基酸序列与大肠杆菌uvrA蛋白的相应序列具有69%的相似性，在螺旋-转角-螺旋、C末端ATP结合和C末端锌指结构域中的相似性分别为90%、100%和85%。透明颤菌血红蛋白基因和uvrA基因3'端之间的距离为63 bp。
