Mormeneo S, Zazueta-Sandoval R, Flores-Carreón A
Departamento de Microbiologia, Facultat de Farmacia, Universitat de Valencia, Spain.
Curr Microbiol. 1995 Apr;30(4):237-41. doi: 10.1007/BF00293639.
Five different fractions containing uronic acids associated with protein were isolated from the cytoplasm of the filamentous form of Mucor rouxii. A single fraction was isolated from the cell wall by hot sodium dodecyl sulfate followed by ion exchange column chromatography. Two cytoplasmic entities (peaks I and II) were not adsorbed to DEAE Bio-Gel A. The molecular mass of peaks I to V ranged from 16.5 to 210 kDa. The protein-uronic acid ratios were different for each fraction. The cell wall fraction showed a molecular mass of 16.5 kDa, similar to that of peak II but with differences in chromatographic behavior and protein-uronic acid ratio. The possible role of these molecules as acceptors of sugar residues during polyuronide chain growth is discussed.
从鲁氏毛霉丝状形式的细胞质中分离出了五种与蛋白质相关的含糖醛酸的不同级分。通过热十二烷基硫酸钠处理，随后进行离子交换柱色谱法，从细胞壁中分离出了一个单一的级分。两个细胞质组分（峰I和峰II）未吸附到DEAE生物凝胶A上。峰I至峰V的分子量范围为16.5至210 kDa。每个级分的蛋白质-糖醛酸比率各不相同。细胞壁级分的分子量为16.5 kDa，与峰II相似，但在色谱行为和蛋白质-糖醛酸比率方面存在差异。讨论了这些分子在聚糖醛酸链生长过程中作为糖残基受体的可能作用。
