Flores-Carreon A, Bartnicki-Garcia S
J Gen Microbiol. 1982 Sep;128(9):2023-7. doi: 10.1099/00221287-128-9-2023.
Cell-free extracts from Mucor rouxii contain enzymes that catalyse the synthesis of uridine diphosphate glucuronic acid (UDPGlcA) from UDPglucose and the incorporation of glucuronic acid from UDPGlcA into polymer(s). Two different polyuronide fraction isolated from the cell walls of this fungus were used as primers. Mucoran, a heteropolymer, was much more efficient than mucoric acid, which is largely a homopolymer of D-glucuronic acid. The primer ability of native cell walls was comparable to that of mucoric acid. Most of the glucuronosyltransferase activity in the cell-free extract was found in a 20000 g particulate fraction. Optimum pH for polyuronide synthesis was 7.0. Mn2+ or Mg2+ stimulated incorporation of GlcA. The products synthesized from mucoric acid and mucoran primers were different and yielded different disaccharides upon hydrolysis.
鲁氏毛霉的无细胞提取物含有一些酶，这些酶可催化由尿苷二磷酸葡萄糖（UDPGlc）合成尿苷二磷酸葡萄糖醛酸（UDPGlcA），并将UDPGlcA中的葡萄糖醛酸掺入聚合物中。从这种真菌的细胞壁中分离出的两种不同的聚糖醛酸级分被用作引物。杂聚物木聚糖比主要为D-葡萄糖醛酸均聚物的木糖酸效率高得多。天然细胞壁的引物能力与木糖酸相当。无细胞提取物中的大部分葡萄糖醛酸基转移酶活性存在于20000g的颗粒级分中。聚糖醛酸合成的最适pH为7.0。Mn2+或Mg2+促进了葡萄糖醛酸（GlcA）的掺入。由木糖酸和木聚糖引物合成的产物不同，水解后产生不同的二糖。
