Das J, Timm H, Busse H G, Degn H
Institute of Biochemistry, University of Kiel, F.R.G.
Yeast. 1990 May-Jun;6(3):255-61. doi: 10.1002/yea.320060310.
The rate of formation of carbon dioxide in cytoplasmic yeast extracts in an open system with continuous infusion of glucose was measured by membrane inlet mass spectrometry during glycolytic oscillations. The rate of CO2 production rose in the first third of each cycle to a maximum of about 100 mumol per ml yeast extract per hour and subsequently diminished to a final level of about 50 mumol per h. Measurements of the NADH light absorption under the same conditions revealed oscillations of relaxation type. The phase of high CO2 production could be related to the phase of the high NADH level, giving evidence that the flux in glycolysis is increased during the phase of high NADH concentration. Only half of the amount of injected glucose was metabolized to CO2 during the sustained oscillation, although free glucose did not accumulate.
在糖酵解振荡过程中，通过膜进样质谱法测量了开放系统中连续输注葡萄糖的细胞质酵母提取物中二氧化碳的生成速率。在每个循环的前三分之一时间内，二氧化碳的产生速率上升至每毫升酵母提取物每小时约100微摩尔的最大值，随后降至约每小时50微摩尔的最终水平。在相同条件下对NADH光吸收的测量显示出弛豫型振荡。高二氧化碳产生阶段可能与高NADH水平阶段相关，这表明在高NADH浓度阶段糖酵解通量增加。在持续振荡过程中，仅一半注入的葡萄糖量被代谢为二氧化碳，尽管游离葡萄糖没有积累。
