一个作为报告基因的儿茶酚2,3-双加氧酶基因。
A catechol 2,3-dioxygenase gene as a reporter.
作者信息
Shindo T, Ueda H, Suzuki E, Nishimura H
机构信息
Department of Chemical Engineering, Faculty of Engineering, University of Tokyo, Japan.
出版信息
Biosci Biotechnol Biochem. 1995 Feb;59(2):314-5. doi: 10.1271/bbb.59.314.
PMID:7766030
Abstract
A catechol 2,3-dioxygenase (C23O) gene of Pseudomonas aeruginosa was expressed under the Simian virus 40 or Rous sarcoma virus promoter in mammalian cells; it was found that the gene could be used as a reporter for the study of gene expression. The C23O gene was a more sensitive reporter than the generally used beta-galactosidase gene.
摘要
铜绿假单胞菌的儿茶酚2,3-双加氧酶（C23O）基因在猿猴病毒40或劳斯肉瘤病毒启动子的调控下于哺乳动物细胞中表达；结果发现该基因可作为研究基因表达的报告基因。C23O基因是一种比常用的β-半乳糖苷酶基因更灵敏的报告基因。
Zotero 插件