Schweizer H P
Department of Microbiology and Infectious Diseases, University of Calgary Health Sciences Center, Alberta, Canada.
Gene. 1993 Nov 30;134(1):89-91. doi: 10.1016/0378-1119(93)90178-6.
Two plasmids, X1918 and Z1918, were constructed which contain the promoter-less xylE and lacZ reporter genes flanked symmetrically by the multiple cloning site (MCS) from pUC19. These cassettes can easily be derived from the multicopy plasmid, pUC1918.
构建了两个质粒,即X1918和Z1918,它们含有无启动子的木糖操纵子基因(xylE)和乳糖操纵子基因(lacZ)报告基因,两侧对称地排列着来自pUC19的多克隆位点(MCS)。这些盒式结构可以很容易地从多拷贝质粒pUC1918中获得。