Teruya K, Yano T, Shirahata S, Watanabe J, Osada K, Ohashi H, Tachibana H, Kim E H, Murakami H
Graduate School of Genetic Resources Technology, Kyushu University, Fukuoka, Japan.
Biosci Biotechnol Biochem. 1995 Feb;59(2):341-4. doi: 10.1271/bbb.59.341.
To rapidly establish recombinant protein hyper-producing cell lines we introduced a reporter plasmid into BHK-21 cells that had been 'primed' by transfection and amplification of the ras oncogene. The reporter plasmid used carries the human interleukin-6 (hIL-6) gene which is under control of the human cytomegalovirus immediate early promoter. The primed BHK cell lines were shown to produce many stable hIL-6 hyper-producing cells achieving about 15 times higher productivity than the control BHK-21 cells.
为了快速建立重组蛋白高产细胞系，我们将一个报告质粒导入经转染和扩增ras癌基因“预处理”的BHK - 21细胞中。所使用的报告质粒携带人白细胞介素 - 6（hIL - 6）基因，该基因受人类巨细胞病毒立即早期启动子的控制。结果表明，预处理的BHK细胞系产生了许多稳定的hIL - 6高产细胞，其生产力比对照BHK - 21细胞高约15倍。
