Shirahata S, Watanabe J, Teruya K, Yano T, Osada K, Ohashi H, Tachibana H, Kim E H, Murakami H
Graduate School of Genetic Resources Technology, Kyushu University, Fukuoka, Japan.
Biosci Biotechnol Biochem. 1995 Feb;59(2):345-7. doi: 10.1271/bbb.59.345.
The amplified ras oncogene greatly enhanced the production of recombinant human interleukin-6 (hIL-6) under control of the cytomegalovirus immediate early promoter (CMV promoter) in BHK-21 cells. When the adenovirus E1A oncogene was further transfected into the above mentioned ras-amplified hIL-6 hyperproducing BHK cells, the transfectants had about 10 times higher productivity than non-transfectants. However, the E1A gene alone did not enhance productivity. These results implicate a ras and E1A synergistic ability that acts to enhance hIL-6 production.
在巨细胞病毒立即早期启动子（CMV启动子）的控制下，扩增的ras癌基因极大地增强了重组人白细胞介素-6（hIL-6）在BHK-21细胞中的产生。当将腺病毒E1A癌基因进一步转染到上述ras扩增的hIL-6高产BHK细胞中时，转染子的生产力比未转染子高约10倍。然而，单独的E1A基因并不能提高生产力。这些结果表明ras和E1A具有协同作用，可增强hIL-6的产生。
